Fig. 7.

Cumate-ribozyme dual control system. (A) Schematic representation of the cumate-ribozyme dual control system: cymR – gene coding for the TetR repressor, cmt – operator sequence of the cumate degradation operon, P21 – synthetic promoter, R – theophylline ribozyme, gusA – reporter gene, Ap^R – ampicillin resistance genes. (B) Glucuronidase activity in cell lysates of recombinant S. albus strains containing gusA under the control of the P21 promoter fused to the cmt operator and theophylline ribozyme in the presence of CymR. The strains were grown in TSB medium for 24 hours. Error bars indicate the standard deviations of triplicate experiments.