Table 1.
Strains and plasmids used in this study.
| Bacterial strains and plasmids | Description | Source or reference |
|---|---|---|
| S. albus J100 | Isoleucine and valine auxotrophic derivative of S. albus G (DSM 40313) lacking SalI-restriction activity | Salas J., Oviedo, Spain |
| S. lividans TK24 | Derivative of S. lividans TK21 that contains mutation in the rpsL gene and is resistant to spectinomycin | (Kieser et al., 2000) |
| E. coli DH5α | routine cloning | MBI Fermentas |
| E. coli ET12567 (pUZ8002) | conjugative transfer of DNA | (Kieser et al., 2000) |
| pUC57 | Apr, general cloning vector | MBI Fermentas |
| pUC19 | Apr, general cloning vector | MBI Fermentas |
| pTOS | Amr; VWB-based integrative vector | (Herrmann et al., 2012) |
| pTOShyg | pTOS with aac(3)IV substituted with hyg | This work |
| pSET152 | Amr; ϕC31-based integrative vector | |
| pHET152 | pSET1529 with aac(3)IV substituted with hyg | This work |
| pKC1139 | Amr; E. coli–Streptomyces shuttle vector | (Kieser et al., 2000) |
| pOJ10700 | HygR; vector containing the hyg gene and oriT flanked by frt sites | (Gust et al., 2004) |
| pGUS | Promoter probe vector containing promoterless gusA | (Myronovskyi et al., 2011) |
| pGUSPA3Ribozyme | Derivative of pGUSbezRBS containing gusA gene fused with HHR and PA3 synthetic promoter | This work |
| pGUSP21Oper | Derivative of pGUS containing the P21 promoter fused to the cmt operator | (Horbal et al., 2014) |
| pGCymRP21 | Derivative of pGUSP21Oper containing the cymR gene | This work |
| pGCymRPA2 | Derivative of pGUS containing PA2 inducible promoter fused with gusA | This work |
| pGCymRPA3 | Derivative of pGUS containing PA3 inducible promoter fused with gusA | This work |
| pGCymRPA4 | Derivative of pGUS containing PA4 inducible promoter fused with gusA | This work |
| pGCymRPA8 | Derivative of pGUS containing PA8 inducible promoter fused with gusA | This work |
| pJETEgfpMcherry | Derivative of pJET1.2, containing egfp under the control of PA3 promoter and the mCherry gene under the control of PA8 | This work |
| pGCymPA3egfpA8RFP | Derivative of pGCymRp21, containing egfp under the control of PA3 promoter and mCherry gene under the control of PA8 | This work |
| pJETA3mcherry | Derivative of pJETEgfpMcherry, containing mCherry gene under the control of PA3 promoter | This work |
| pJETA3cherryA8gfp | Derivative of pJETEgfpMcherry, containing the mCherry gene under the control of PA3 promoter and egfp under – PA8 | This work |
| pGCymRPA2A3RFPA8gfp | Derivative of pGCymRp21, containing egfp under the control of PA8 promoter and the mCherry gene under the control of PA3 | This work |
| pGUST_ermE_E* | Derivative of pGUS containing gusA gene fused with theophylline riboswitch | |
| pGCymRibos | Derivative of pGCymRP21, containing the gusA gene under the control of P21-cmt promoter and theophylline riboswitch | This work |
| pGUSRolRibos | Derivative of pGUSRolRPA3, containing the gusA gene under the control of PA3-rolO promoter and theophylline riboswitch | This work |
| pGUSPA3Ribozyme | Derivative of pGUSbezRBS containing gusA gene fused with HHR and PA3 synthetic promoter | (Horbal et al., 2014) |
| pGCymRibozyme | Derivative of pGUSRolRPA3, containing the gusA gene under the control of P21-cmt promoter and theophylline HHR | This work |
| pALCre | pAL1 derivative with cre(a) gene under tipA promoter | (Fedoryshyn et al., 2008) |
| pKCHygCymRibos | Derivative of pKH1139, containing the gusA gene under the control of P21-cmt promoter and theophylline riboswitch | This work |
| pH1139 | pKC1139 with aac(3)IV substituted with hyg | Myronovskyi et al., 2014 |
| pKHygCymRibosCre | Derivative of pKH1139, containing the cre gene under the control of P21-cmt promoter and theophylline riboswitch | This work |
| pCreInt | Derivative of pSET152, containing aac(3)IV surrounded with direct loxP sites | (Fedoryshyn et al., 2008) |
| pGCymRibosCre | Derivative of pGCymRibos, containing the cre gene under the control of P21-cmt promoter and theophylline riboswitch | This work |
| pGRolRibosCre | Derivative of pGUSRolRibos, containing the cre gene under the control of PA3-rolO promoter and theophylline riboswitch | This work |
| pGusP21Riboswitch | Derivative of pGCymRibos, containing the gusA gene under the control of P21 promoter and theophylline riboswitch | This work |
| pKCHygP21Riboswitch | Derivative of pKHygCymRibos, containing the gusA gene under the control of P21 promoter and theophylline riboswitch | This work |
| pKCHygCymRibosPamJ | Derivative of pKHygCymRibos, containing the pamJ gene under the control of P21-cmt promoter, CymR and theophylline riboswitch | This work |
| pTOShyg | Derivative of pTOS with aac(3)IV substituted with hyg | This work |
| pTOShygCymRibosCre | Derivative of pTOShyg, containing the cre gene under the control of P21-cmt promoter and theophylline riboswitch | This work |
| pTOShygRolRibosCre | Derivative of pTOShyg, containing the cre gene under the control of PA3-rolO promoter and theophylline | This work |