Table 1.
Primers developed for multiplex PCR used to amplify the matK barcoding region. The forward (C_MATK_F) and reverse (C_MATK_R) primer cocktail as well as the four additional primers are given with their proportions in the primer cocktail.
| Cocktail name/Primer name (Direction) | Proportion in primer cocktail | Primer sequence (5′–3′)a | Primer positionb |
| C_MATK_F | 383–413 | ||
| matK-413f-1 (Forward) | 2 | TAATTTACRATCAATTCATTCAATATTTCC | |
| matK-413f-2 (Forward) | 2 | TAATTTACGATCYATTCATTCAATATTTCC | |
| matK-413f-3 (Forward) | 1 | TAATTTACGATCAATTCATTCAACATTTCC | |
| matK-413f-4 (Forward) | 2 | TAATTTMCRATCAATTCATTCCATATTTCC | |
| matK-413f-5 (Forward) | 1 | TAATTTACGATCAATTCATTCACTATTTCC | |
| C_MATK_R | 1227–1256 | ||
| matK-1227r-1 (Reverse) | 3 | GARGAYCCRCTRTRATAATGAGAAAGATTT | |
| matK-1227r-2 (Reverse) | 1 | GAAGAYCCGCTATGATAATGAGAAAGGTTT | |
| matK-1227r-3 (Reverse) | 2 | GARGATCCRCTRTRATAATGAAAAAGATTT | |
| matK-1227r-4 (Reverse) | 2 | GARGATCCRCTRTRATAATGAGAAAAATTT | |
| matK-1227r-5 (Reverse) | 2 | GARGATCCRCTRTRATAATGAGAAATATTT | |
| Additional primers | |||
| matK-413f-6 (Forward) | 2 | TAATTTACGATCWATTCATTCMATTTTTCC | 383–413 |
| matK-413f-7 (Forward) | 1 | TAATTTACAATCMATTCATTCAATATTTTC | 383–413 |
| matK-1227r-6 (Reverse) | 2 | GARGATCCGCTRTAATAATGCGAAAGATTT | 1227–1256 |
| matK-1227r-7 (Reverse) | 2 | GARGATCCGCTATRATAATGATAAATATTT | 1227–1256 |
a
Ambiguous bases are set in boldface.
b
Primer position is given for Arabidopsis thaliana (GenBank accession no. AF144378.1).