Table 2.
Recommended use of primers for different families, based on BLAST matches with no mismatches.a
| Order | Family | Appropriate forward primer | Appropriate reverse primer |
| Alismanthales | Alismataceae | matK-413f-2 | matK-1227r-1, matK-1227r-3 |
| Araceae | matK-413f-2, matK-413f-5 | matK-1227r-1 | |
| Apiales | Araliaceae | matK-413f-2, matK-413f-5 | matK-1227r-1, matK-1227r-4 |
| Apiaceae | matK-413f-7 | matK-1227r-1, matK-1227r-5 | |
| Aquifoliales | Aquifoliaceae | matK-413f-1 | matK-1227r-1, matK-1227r-3 |
| Cardiopteridaceae (Gonocaryum minus) | matK-413f-1 | matK-1227r-1, matK-1227r-3 | |
| Stemonuraceae | matK-413f-1 | matK-1227r-1, matK-1227r-3 | |
| Arecales | Arecaceae (Arecaceae sp.) | matK-413f-2 | matK-1227r-1, matK-1227r-3 |
| Asparagales | Amaryllidaceae | matK-413f-6 | matK-1227r-1, matK-1227r-3 |
| Asparagaceae | matK-413f-6 | matK-1227r-1, matK-1227r-4, | |
| matK-1227r-5 | |||
| Hyacinthaceae | matK-413f-6 | matK-1227r-1, matK-1227r-3 | |
| Iridaceae | matK-413f-6 | matK-1227r-1, matK-1227r-3, | |
| matK-1227r-5 | |||
| Orchidaceae (Polystachya humbertii) | matK-413f-1, matK-413f-2, | matK-1227r-1, matK-1227r-2, | |
| matK-413f-3, matK-413f-6 | matK-1227r-3 | ||
| Tecophilaeaceae | matK-413f-6 | matK-1227r-1 | |
| Xanthorrhoeaceae | matK-413f-6 | matK-1227r-1, matK-1227r-5 | |
| Asterales | Asteraceae (Leontodon hispidus) | matK-413f-1 | matK-1227r-1, matK-1227r-2, |
| matK-1227r-3, matK-1227r-4, | |||
| matK-1227r-5 | |||
| Campanulaceae | matK-413f-2 | matK-1227r-1,matK-1227r-5 | |
| Goodeniaceae | matK-413f-4 | matK-1227r-1 | |
| Austrobaileyales | Austrobaileyaceae | matK-413f-2 | matK-1227r-2 |
| Schisandraceae | matK-413f-2 | matK-1227r-2 | |
| Trimeniaceae | matK-413f-2 | matK-1227r-2 | |
| Berberidopsidales | Berberidopsidaceae | matK-413f-1 | matK-1227r-1 |
| Boraginales | Boraginaceae | matK-413f-1, matK-413f-4 | matK-1227r-1, matK-1227r-3, |
| matK-1227r-5 | |||
| Ehretiaceae | matK-413f-1 | matK-1227r-1 | |
| Brassicales | Brassicaceae | matK-413f-1, matK-413f-4, matK-413f-6 | matK-1227r-1, matK-1227r-5 |
| Capparaceae | matK-413f-1 | matK-1227r-1 | |
| Caricaeae | matK-413f-1 | matK-1227r-1 | |
| Cleomaceae | matK-413f-1, matK-413f-3, | matK-1227r-1, matK-1227r-2, | |
| matK-413f-4, matK-413f-7 | matK-1227r-4, matK-1227r-5 | ||
| Moringaceae | matK-413f-1 | matK-1227r-1, matK-1227r-5 | |
| Resedaceae | matK-413f-1 | matK-1227r-1 | |
| Bruniales | Brunelliaceae | matK-413f-1 | matK-1227r-1 |
| Buxales | Bucaceae | matK-413f-1 | matK-1227r-1 |
| Caryophyllales | Amaranthaceae | matK-413f-1 | matK-1227r-1 |
| Cactaceae | matK-413f-1 | matK-1227r-1 | |
| Polygonaceae | matK-413f-1 | matK-1227r-1, matK-1227r-2, | |
| matK-1227r-5 | |||
| Simmondsiaceae | matK-413f-1 | matK-1227r-3 | |
| Tamaricaceae | matK-413f-1 | matK-1227r-1 | |
| Celastrales | Celastraceae | matK-413f-1, matK-413f-4, | matK-1227r-1, matK-1227r-2, |
| matK-413f-6 | matK-1227r-3, matK-1227r-4, | ||
| matK-1227r-5 | |||
| Lepidobotryaceae | matK-413f-1 | matK-1227r-5 | |
| Chloranthales | Chloranthaceae | matK-413f-2 | matK-1227r-1, matK-1227r-5 |
| Commelinales | Commelinaceae | matK-413f-2 | matK-1227r-1 |
| Haemodoraceae | matK-413f-2 | matK-1227r-1, matK-1227r-2, | |
| matK-1227r-5 | |||
| Cornales | Cornaceae (Alangium cf. javanicum, Mastixia sp.) | matK-413f-1, matK-413f-3 | matK-1227r-1, matK-1227r-3, |
| matK-1227r-4, matK-1227r-5 | |||
| Grubbiaceae | matK-413f-1 | matK-1227r-1 | |
| Hydrangeaceae | matK-413f-1 | matK-1227r-1, matK-1227r-4 | |
| Loasaceae | matK-413f-1, matK-413f-7 | matK-1227r-1, matK-1227r-4 | |
| Crossosomatales | Stachyuraceae | matK-413f-1 | matK-1227r-1 |
| Staphyleaceae | matK-413f-1 | matK-1227r-1, matK-1227r-5 | |
| Strasburgeriaceae | matK-413f-1 | matK-1227r-1 | |
| Cucurbitales | Anisophylleaceae (Anisophyllea sp.) | matK-413f-1, matK-413f-6 | matK-1227r-1 |
| Begoniaceae | matK-413f-1, matK-413f-6 | matK-1227r-1 | |
| Coriariaceae | matK-413f-2 | matK-1227r-1 | |
| Cucurbitaceae | matK-413f-2 | matK-1227r-1, matK-1227r-3, | |
| matK-1227r-4, matK-1227r-5 | |||
| Datiscaceae | matK-413f-1 | matK-1227r-1 | |
| Tetramelaceae | matK-413f-1 | matK-1227r-3, matK-1227r-5 | |
| Dipsacales | Adoxaceae | matK-413f-4 | matK-1227r-1 |
| Caprifoliaceae | matK-413f-1, matK-413f-5 | matK-1227r-1 | |
| Ericales | Ebenaceae (Diospyros sp.) | matK-413f-1 | matK-1227r-1, matK-1227r-3, |
| matK-1227r-6 | |||
| Ericaceae | matK-413f-1, matK-413f-4 | matK-1227r-1, matK-1227r-5 | |
| Lecythidaceae (Barringtonia curranii) | matK-413f-5 | matK-1227r-1 | |
| Pentaphylacaceae | matK-413f-1 | matK-1227r-1 | |
| Primulaceae (Ardisia sp.) | matK-413f-1, matK-413f-2 | matK-1227r-3, matK-1227r-1, | |
| matK-1227r-5, matK-1227r-7 | |||
| Styracaceae | matK-413f-1 | matK-1227r-1 | |
| Symplocaceae (Symplocos crassipes) | matK-413f-1 | matK-1227r-1 | |
| Theaceae | matK-413f-1 | matK-1227r-4 | |
| Escalloniales | Escalloniaceae | matK-413f-1 | matK-1227r-1 |
| Fabales | Fabaceae (Fordia splendidissima) | matK-413f-1, matK-413f-2, | matK-1227r-1, matK-1227r-3, |
| matK-413f-4, matK-413f-6, | matK-1227r-5 | ||
| matK-413f-7 | |||
| Polygalaceae (Xanthophyllum beccarianum) | matK-413f-1, matK-413f-2 | matK-1227r-1 | |
| Fagales | Betulaceae | matK-413f-2 | matK-1227r-1 |
| Casuarinaceae | matK-413f-2 | matK-1227r-1 | |
| Fagaceae (Lithocarpus sp.) | matK-413f-2 | matK-1227r-1, matK-1227r-3, | |
| matK-1227r-5 | |||
| Juglandaceae | matK-413f-1 | matK-1227r-1, matK-1227r-6 | |
| Garryales | Garryaceae | matK-413f-1 | matK-1227r-1, matK-1227r-4, |
| matK-1227r-6 | |||
| Gentianales | Apocynaceae (Tabernaemontana sp.) | matK-413f-1, matK-413f-3, | matK-1227r-1, matK-1227r-2, |
| matK-413f-4, matK-413f-5, | matK-1227r-6 | ||
| matK-413f-6 | |||
| Loganiaceae | matK-413f-1 | matK-1227r-1, matK-1227r-5 | |
| Rubiaceae (Urophyllum sp., Psychotria sp.) | matK-413f-1, matK-413f-5 | matK-1227r-1, matK-1227r-2 | |
| Geraniales | Geraniaceae | matK-413f-1, matK-413f-6 | matK-1227r-1 |
| Melianthaceae | matK-413f-1, matK-413f-6 | matK-1227r-1 | |
| Gunnerales | Gunneraceae | matK-413f-1, matK-413f-2 | matK-1227r-1 |
| Huerteales | Dipentodontaceae | matK-413f-1 | matK-1227r-1 |
| Gerrardinaceae | matK-413f-1 | matK-1227r-1 | |
| Tapisciaceae | matK-413f-1 | matK-1227r-1, matK-1227r-5 | |
| Icacinales | Icacinaceae | matK-413f-1 | matK-1227r-1, matK-1227r-3 |
| Lamiales | Acanthaceae | matK-413f-1 | matK-1227r-1, matK-1227r-2, |
| matK-1227r-4, matK-1227r-5 | |||
| Gesneriaceae | matK-413f-1 | matK-1227r-1, matK-1227r-2, | |
| matK-1227r-5 | |||
| Lamiaceae (Teijsmanniodendron sp.) | matK-413f-1 | matK-1227r-1, matK-1227r-2, | |
| matK-1227r-5 | |||
| Lentibulariaceae | matK-413f-1 | matK-1227r-1 | |
| Myrsinaceae | matK-413f-1 | matK-1227r-1 | |
| Oleaceae | matK-413f-1 | matK-1227r-1, matK-1227r-2, | |
| matK-1227r-3, matK-1227r-4 | |||
| Orobanchaceae | matK-413f-1 | matK-1227r-1, matK-1227r-3, | |
| matK-1227r-4 | |||
| Laurales | Hernandiaceae | matK-413f-2 | matK-1227r-1 |
| Lauraceae (Litsea sarawacensis) | matK-413f-2 | matK-1227r-1, matK-1227r-3 | |
| Siparunaceae | matK-413f-2 | matK-1227r-3 | |
| Liliales | Smilacaceae | matK-413f-2 | matK-1227r-1, matK-1227r-5 |
| Magnoliales | Annonaceae | matK-413f-2 | matK-1227r-1, matK-1227r-4, |
| matK-1227r-5 | |||
| Degeneriaceae | matK-413f-2 | matK-1227r-1 | |
| Eupomatiaceae | matK-413f-2 | matK-1227r-1 | |
| Himantandraceae | matK-413f-2 | matK-1227r-1 | |
| Magnoliaceae (Magnolia sp.) | matK-413f-2, matK-413f-6 | matK-1227r-1 | |
| Myristicaceae | matK-413f-2, matK-413f-4 | matK-1227r-1, matK-1227r-5 | |
| Malpighiales | Clusiaceae (Garcinia sp.) | matK-413f-1 | matK-1227r-1, matK-1227r-3, |
| matK-1227r-4, matK-1227r-5 | |||
| Euphorbiaceae | matK-413f-1 | matK-1227r-1, matK-1227r-3, | |
| (Antidesma sp., Drypetes sp., Koilodepas sp., Macaranga hosei, Mallotus sp.) | matK-1227r-4, matK-1227r-5 | ||
| Linaceae | matK-413f-1 | matK-1227r-1 | |
| Passifloraceae | matK-413f-1 | matK-1227r-1 | |
| Phyllanthaceae | matK-413f-1, matK-413f-2, | matK-1227r-1 | |
| matK-413f-7 | |||
| Putranjivaceae | matK-413f-1 | matK-1227r-5 | Rhizophoraceae |
| matK-413f-5 | matK-1227r-1, matK-1227r-3 | Salicaceae | |
| matK-413f-1 | matK-1227r-1, matK-1227r-5 | Violaceae (Rinorea sp.) | |
| matK-413f-1 | matK-1227r-1, matK-1227r-6 | Malvales | |
| Elaeocarpaceae | matK-413f-1 | ||
| matK-1227r-1 | Malvaceae (Durio griffithii, Leptonychia sp., Sterculia sp.) | matK-413f-1 | matK-1227r-1 |
| Myrtales | Lythraceae | matK-413f-1, matK-413f-5 | matK-1227r-1, matK-1227r-3 |
| Melastomataceae | matK-413f-7 | matK-1227r-1, matK-1227r-4 | |
| Myrtaceae (Syzygium sp.) | matK-413f-1, matL-413f-4, | matK-1227r-1, matK-1227r-3, | |
| matK-413f-6 | matK-1227r-4, matK-1227r-5 | ||
| Onagraceae | matK-413f-3 | matK-1227r-1 | |
| Oxalidales | Brunelliaceae | matK-413f-1 | |
| Cunoniaceae | matK-413f-1 | matK-1227r-1 | |
| Huaceae | matK-413f-6 | matK-1227r-1 | |
| Pandanales | Cyclanthaceae | matK-413f-2 | matK-1227r-1 |
| Pandanaceae | matK-413f-2 | matK-1227r-1 | |
| Paracryphiales | Paracryphiaceae | matK-413f-1 | matK-1227r-1 |
| Piperales | Aristolochiaceae | matK-413f-2 | matK-1227r-1, matK-1227r-5 |
| Piperaceae | matK-413f-2 | matK-1227r-3 | |
| Saururaceae | matK-413f-2 | matK-1227r-1 | |
| Poales | Bromeliaceae (Tillandsia cf. caloura) | matK-413f-2, matK-413f-6 | matK-1227r-1, matK-1227r-3 |
| Typhaceae | matK-413f-2 | matK-1227r-1, matK-1227r-3 | |
| Proteales | Nelumbonaceae | matK-413f-1 | matK-1227r-1 |
| Platanaceae | matK-413f-1 | matK-1227r-1 | |
| Proteaceae | matK-413f-1, matK-413f-2, | matK-1227r-1, matK-1227r-3, | |
| matK-413f-3 | matK-1227r-4, matK-1227r-5 | ||
| Ranunculales | Berberidaceae | matK-413f-3 | matK-1227r-1 |
| Eupteleaceae | matK-413f-1, matK-413f-2 | matK-1227r-1 | |
| Lardizabalaceae | matK-413f-1 | matK-1227r-1, matK-1227r-5 | |
| Papaveraceae | matK-413f-1, matK-413f-2, | matK-1227r-1, matK-1227r-3, | |
| matK-413f-3, matK-413f-5 | matK-1227r-5 | ||
| Ranunculaceae | matK-413f-4 | matK-1227r-1, matK-1227r-6, | |
| matK-1227r-4, matK-1227r-5 | |||
| Rosales | Cannabaceae (Gironniera nervosa) | matK-413f-1, matK-413f-3 | matK-1227r-1, matK-1227r-3 |
| Moraceae (Artocarpus elasticus) | matK-413f-1 | matK-1227r-3 | |
| Rhamnaceae (Ziziphus angustifolius) | matK-413f-1, matK-413f-7 | matK-1227r-1, matK-1227r-3 | |
| Rosaceae | matK-413f-1, matK-413f-2, | matK-1227r-1, matK-1227r-3, | |
| matK-413f-6 | matK-1227r-4, matK-1227r-5 | ||
| Ulmaceae | matK-413f-1 | matK-1227r-3 | |
| Urticaceae | matK-413f-1 | matK-1227r-3 | |
| Sabilales | Sabiaceae (Meliosma sumatrana) | matK-413f-1, matK-413f-2 | matK-1227r-1, matK-1227r-4 |
| Santalales | Loranthaceae | matK-413f-4 | matK-1227r-1, matK-1227r-4 |
| Opiliaceae | matK-413f-1, matK-413f-2 | matK-1227r-1 | |
| Santalaceae | matK-413f-1, matK-413f-2 | matK-1227r-1, matK-1227r-5 | |
| Schoepfiaceae | matK-413f-1 | matK-1227r-1, matK-1227r-4 | |
| Sapindales | Meliaceae (Aglaia sp.) | matK-413f-1, matK-413f-7 | matK-1227r-1, matK-1227r-5 |
| Rutaceae (Glycosmis macrantha) | matK-413f-1 | matK-1227r-1, matK-1227r-6, | |
| matK-1227r-5 | |||
| Sapindaceae (Lepisanthes sp.) | matK-413f-4 | matK-1227r-1, matK-1227r-3, | |
| matK-1227r-5 | |||
| Saxifragales | Cercidiphyllaceae | matK-413f-1, matK-413f-7 | matK-1227r-1 |
| Haloragaceae | matK-413f-1 | matK-1227r-1 | |
| Hamamelidaceae | matK-413f-1, matK-413f-5 | matK-1227r-1 | |
| Paeoniaceae | matK-413f-1 | matK-1227r-1 | |
| Saxifragaceae | matK-413f-1, matK-413f-4, | matK-1227r-1 | |
| matK-413f-5 | |||
| Solanales | Montiniaceae | matK-413f-1 | matK-1227r-1 |
| Solanaceae | matK-413f-1, matK-413f-3 | matK-1227r-3 | |
| Trochodendrales | Trochodendraceae | matK-413f-1, matK-413f-6 | matK-1227r-1 |
| Vitales | Vitaceae | matK-413f-1 | matK-1227r-1, matk-1227r-2, |
| matK-1227r-5 |
a
Species/genera in parentheses were successfully amplified in the family using the primer cocktail C_MATK_F/C_MATK_R.