Table 3.

Transferability of the 22 EST-SSR markers for Japanese Aster and Solidago species.

Locus	A. ageratoides var. ageratoides (N = 3)a	A. glehnii var. hondoensis (N = 2)b	A. hispidus var. tubulosus (N = 2)b,c	A. rugulosus (N = 6)a	A. scaber (N = 3)b	A. sohayakiensis (N = 2)b	S. virgaurea subsp. asiatica var. asiatica (N = 4)a	A. savatieri (Nagano population) (N = 2)b,d	A. savatieri var. pygmaeus (Kochi population) (N = 2)b,d
Ast_comp41702_c0_seq1	—	+	+	—	+	—	+	+	—
Ast_comp53978_c4_seq1	—	+	—	+	+	—	+	+	+
Ast_comp54189_c0_seq15	+	NG	NG	+	—	+	NG	+	—
Ast_comp22325_c0_seq1	NG	—	+	—	—	—	—	+	—
Ast_comp37017_c0_seq1	+	+	+	+	+	—	+	—	+
Ast_comp36481_c0_seq1	+	—	+	—	+	—	+	+	+
Ast_comp55030_c0_seq87	+	+	+	+	+	—	NG	—	+
Ast_comp41314_c0_seq1	NG	—	NG	NG	NG	NG	+	—	+
Ast_comp48897_c0_seq1	+	NG	+	NG	+	+	NG	+	+
Ast_comp51216_c2_seq2	+	—	+	+	+	+	NG	+	+
Ast_comp50838_c2_seq2	NG	—	—	+	—	+	+	NG	—
Ast_comp55875_c0_seq1	—	+	NG	—	—	NG	+	+	+
Ast_comp53959_c2_seq2	—	NG	+	+	—	NG	+	—	+
Ast_comp46752_c1_seq1	—	+	—	+	+	+	+	—	+
Ast_33509	+	+	+	—	+	—	—	+	+
Ast_19559	—	NG	NG	+	+	NG	+	+	+
Ast_44410	—	NG	NG	+	+	+	NG	+	+
Ast_65237	+	+	+	+	+	+	+	+	+
Ast_47436	NG	NG	NG	NG	NG	NG	NG	—	—
Ast_34501	+	NG	+	+	—	+	+	—	—
Ast_59032	+	NG	+	+	—	—	+	—	+
Ast_26109	+	—	NG	—	+	—	+	+	+
No. of successfully amplified loci	18	14	15	19	20	17	16	21	22

Note: — = monomorphic (only one allele was detected); + = polymorphic (more than one allele was detected); NG = no signal or nonspecific amplification was detected in PCR amplification.

^aIndividuals originated from more than one population.

^bIndividuals originated from a single population.

^cPutative tetraploid.

^dSamples used for initial PCR amplification trials.