Table A1.
Costs associated with microsatellite development for 12–15 loci and RAD/GBS costs to generate thousands of loci. Both budgets assume 96 individuals are included in the study.
| Item | Base cost | Quantity | Total cost |
| Microsatellites | |||
| QIAGEN PCR multiplex kit | $270 | 4 | $1080 |
| Unlabeled primer pairs | $6 | 48 | $288 |
| Labeled primer (single) | $80 | 24 | $1920 |
| Genotyping one plate | $100 | 4 | $400 |
| Total | $3688 | ||
| RAD/GBS (high estimate) | |||
| Double digest | $1261 | 1 | $1261 |
| Digest optimization | $539 | 1 | $539 |
| QC Bioanalyzer chips | $101 | 10 | $1010 |
| Qubit quantification | $400 | 1 | $400 |
| Illumina HiSeq lane | $1225 | 1 | $1225 |
| dsDNA Fluorophore quantification | $69 | 1 | $69 |
| Reagents | $500 | 1 | $500 |
| Total | $5004 | ||
| RAD/GBS (low estimate) | |||
| Per sample cost | $31.92 | 96 | $3064.32 |
| Fixed cost | $340 | 1 | $340 |
| Total | $3404.32 |