Table I.
Primers used for conventional RT-PCR.
| Gene | Forward | Reverse | Tm | Product size (bp) |
|---|---|---|---|---|
| VEGFR1 | AGTTTAAAAGGCACCCAGCA | ACGAGCTCCCTTCCTTCAGT | 55 | 362 |
| VEGFR2 | GAGGGACTTGGACTGGCTTT | GATTTGAAATGGACCCGAGA | 55 | 302 |
| VEGFR3 | TGAACATCACGGAGGAGTCA | TCAGGCTTGTTGATGAATGG | 55 | 337 |
| NRP1 | GAAAAATGCGAATGGCTGAT | AATCCGGGGGACTTTATCAC | 53 | 335 |
| NRP2 | CAAACACTGTGGGAACATCG | TGTCCAGCCAATCGTACTTG | 55 | 338 |
| DLL1 | TGTGCCTCAAGCACTACCAG | ACACACGAAGCGGTAGGAGT | 55 | 353 |
| DLL4 | TATTGGGCACCAACTCCTTC | ACATAGTGGCCGAAGTGGTC | 55 | 349 |
| NOTCH 1 | GGCCAGAACTGTGAGGAAAA | GCAGTAGAAGGAGGCCACAC | 57 | 327 |
| NOTCH 4 | TCTCCCTCTCCATTGACACC | TGGAAGCACTCGTTGACATC | 55 | 323 |
| HEY2 | TGGGGAGCGAGAACAATTAC | GCACTCTCGGAATCCTATGC | 55 | 329 |
| EFNB2 (C) | TTATTTGCCCCAAAGTGGAC | CCTGGTTGATCCAGCAGAAC | 55 | 347 |
| EPHB4 | GAGCTGTGTGGCAATCAAGA | ACTTTGCAGACGAGGTTGCT | 55 | 345 |
| COUP TFII | AACACATCGAGTGCGTGGT | CAGGTACGAGTGGCAGTTGA | 55 | 311 |
| PECAM1 | GCAAAATGGGAAGAACCTGA | ACAGTTGACCCTCACGATCC | 55 | 316 |
| VECAD | TGGACAAGGACACTGGTGAA | TCTTGCAGAGTGACCAGCAC | 55 | 382 |
| ACTB | GCCAGCTCACCATGGATGAT | GTCTCAAACATGATCTGGGTC | 57 | 388 |