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. 2016 Jun 3;8:470–473. doi: 10.1016/j.dib.2016.05.073
Subject area Biology
More specific subject area Protein expression in Mytilus edulis
Type of data Table,.xlsx file
How data was acquired Data dependent LC-MS/MS acquired on a Q Exactive instrument (Thermo Fisher Scientific) coupled to a ultra nanoflow high-performance liquid chromatography (HPLC) system (EASY-nLC™ 1000, Thermo Fisher Scientific)
Data format Processed, analyzed
Experimental factors
  • a)

    Lipopolysaccharide stimulation of blue mussels and protein extraction from gills

  • a)

    LC-MS/MS analysis for protein identification and quantification

Experimental features Blue mussels were stimulated with lipopolysaccharides and samples were collected at different time points post injection. Protein extracts were prepared from the gills, digested using trypsin and a full in-depth proteome investigation was performed using LC-Orbitrap MS/MS technique. The spectra (.RAW) were acquired using Xcalibur software 3.0.63 and further database searches were performed using MaxQuant 1.5.1.2. The search results were stored as.xls-files.
Data source location Uppsala, Sweden
Data accessibility Data are within this article (Supplementary Table 1)