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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Int Immunopharmacol. 2016 Jan 8;37:31–42. doi: 10.1016/j.intimp.2015.12.034

Figure 3. IL-6 expression is downregulated by mouse macrophages following exposure to 10 mM creatine monohydrate.

Figure 3

Immunohistochemical staining demonstrated a decrease in IL-6 expression following incubation with 10 mM creatine monohydrate for 24 (B) or 48 (C) hours. Control (A) cells were incubated with media alone. Non-specific staining controls received rat IgG (isotype control) in lieu of primary antibody (D). Immunohistochemical staining for IL-6 was performed using the Avidin Biotin Complex (ABC) method (Vector Laboratories) as previously described [238] using an antibody to mouse IL-6 (BD Pharmingen). Development was performed using the AEC Substrate Kit (Vector Laboratories). Positive staining is indicated by the brown reaction product.