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Inhibition of IGFBP2 impairs oncogenic signalling pathways. A. U251 cells were serum starved overnight and incubated with increasing doses of IGFBP2-neutralizing antibody (nAb). A Western blot analysis was performed on whole cell extracts (WCEs). B. U87MG cells were serum starved overnight and incubated with rhIGFBP2 for the indicated times. Western blot analyses were performed on WCEs and isolated histone fractions.
