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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Neurochem. 2016 Jun 6;138(1):101–116. doi: 10.1111/jnc.13643

Figure 4. Effect of Quercetin and other flavonoids on OGD and OGD/ROG-induced cell death in SHSY5Y cells and rat cortical neurons.

Figure 4

(A) SHSY5Y cells or rat cortical neurons were subjected to OGD for 16 hr (SHSY5Y) or 5 hr (cortical neurons) in the presence of 10 μM of various flavonoids with or without pretreatment (10 μM for 6 hr for SHSY5Y and 3 hr for cortical neurons) as indicated. Cell death was measured via LDH release and is compared to DMSO alone. Upper panel: SHSY5Y cells; lower panel: cortical neurons. (B) SHSY5Y cells or rat cortical neurons were subjected to OGD for 16 hr (SHSY5Y) or 5 hr (cortical neurons) in the presence of various concentrations of quercetin (0, 10, 20, 40 μM). After OGD cell viability was measured using a premixed WST-1 cell proliferation reagent. Cell viability was shown as the percentage of untreated (no OGD, no quercetin added) cells. Left panel: SHSY5Y cells; Right panel: cortical neurons. (C) Cell death after OGD/ROG was measured via LDH release and is shown as the percentage of total LDH. Left panel: SHSY5Y; Right panel: cortical neurons. Data represent the mean ± standard deviation of at least three independent experiments. ***p<0.001, **p<0.01, *p<0.05 compared to the DMSO alone control via a one way ANOVA, followed by Dunnett’s post-hoc test.