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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Neurochem. 2016 Jun 6;138(1):101–116. doi: 10.1111/jnc.13643

Figure 5. Quercetin induced cellular protection against OGD/ROG is SUMOylation dependent.

Figure 5

Rat B35 neuroblastoma cell lines stably expressing miR negative (Y168), miR-negative with SUMO-1 shRNA#2, SUMO-2,3 microRNA (Y241) alone, and SUMO-2,3 miRNA with SUMO-1 shRNA#2 were incubated with quercetin at 20 and 40 μM for 16 hr, and the levels of SUMO-conjugation and cellular tolerance to OGD/ROG were examined. (A) Representative immunoblots (upper panel) and their quantitative analyses (lower panel). (B) Cell death after OGD/ROG (for 16hr/5hr respectively) was measured via LDH release and is shown as percentages of control (Y168 cells without quercetin, DMSO alone). Data represent the mean ± standard deviation of at least three independent experiments. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05 compared to the DMSO alone control via a one way ANOVA, followed by Dunnett’s post-hoc test.