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. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Oncogene. 2015 Dec 21;35(31):4091–4101. doi: 10.1038/onc.2015.471

Figure 5.

Figure 5

Fyn deficiency confers resistance against SSL-induced apoptosis. (A) MEFs and (B) HaCaT cells deficient in Fyn are resistant to SSL-induced apoptosis. Apoptosis was determined by flow cytometry as described in Methods. SSL-induced apoptotic signaling is impaired in skin samples from Fyn−/− mice compared to wildtype animals (C). Fyn−/− MEFs (D) and shFyn HaCaT cells (E) also impaired SSL-induced apoptotic signaling compared to wt or Mock cells. Cells were exposed to SSL and harvested at the indicated time points for Western blot analysis using specific antibodies. Western blots are representative of similar results from duplicate experiments. Fyn−/− MEFs (F) and HaCaT cells (G) transduced with wt or mutant Fyn (C488A). Cells were exposed to SSL and harvested at 12 h. Apoptosis was determined by flow cytometry as described in Materials and Methods. T and C indicate the total and cleaved forms, respectively. Data are shown as means ± S.D. from duplicate experiments. The asterisks (**) indicate a significant difference (p < 0.01) between Fyn+/+ and Fyn−/− MEFs or mock-transfected and shFyn-expressing HaCaT cells.