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a Low magnification (×4) of control group. b Low magnification (×4) of vitamin D deficiency group. c Higher magnification (×10) of control group. Note the red Safranin O stain indicating proteoglycans in the cartilage matrix. d In this higher magnification (×10) of vitamin D deficiency group, the loss of proteoglycans is evident by the loss of red Safranin O staining.
