FAP-TAPs induced photo-ablation of cardiac function. (a) Phenotype development
from 0 hpi to 96 hpi of larval zebrafish (Merge of DIC and mCer3 fluorescence
(cyan), n = 20 for each group):
MG-ester/Tgpt22,
MG-2I/Tgpt22 and MG-2I/WT. In
MG-2I/Tgpt22 group, the larvae developed a
range of visible defects: large cardiac edema, small eyes, and collapsed,
nonfunctional heart chambers. In both control groups, development proceeded
normally. Scale Bar = 1000 μm and applied to all images; (b)
FAP-TAPs photo-induced cardiac damage in adult zebrafish. Hearts were extracted
at 3 dpi and TUNEL was performed to assess cell death; (c) Acid Fuchsin Orange G
(AFOG) staining of hearts at 5 dpi showing the damaged cardiac structure in
transgenic fish injected with MG-2I; (d) Mef2c (cardiomyocyte) and PCNA
(proliferation) staining at 5 dpi shows enhanced cardiomyocyte proliferation
(yellow arrow) in transgenic fish injected with MG-2I. Scale bar = 100
μm and applied to all images, n = 9 for all groups, One-way
ANOVA, Tukey post hoc tests were performed with multiple comparisons of mean for
each group. P-values were considered significant when <
0.05, shown as mean ± S.E.M.