Table 1.
Bacterial strains and plasmids used in the present study.
| Strain/plasmid | Characteristics | Reference/source |
|---|---|---|
| Strains | ||
| Escherichia coli DH10B | Cloning host | Thermo Fisher Scientific |
| E. coli BL21 DE3 | Used for protein overexpression | New England Biolabs |
| E. coli BL21 DE3 pET29a_agrB | IPTG-inducible expression of agrB, Kanr | This study |
| E. coli BL21 DE3 pET29a_agrBD | IPTG-inducible expression of agrBD, Kanr | This study |
| Listeria monocytogenes EGD-e | Bécavin et al., 2014 | |
| L. monocytogenes ΔagrD | In-frame deletion of agrD in strain EGD-e | Riedel et al., 2009 |
| L. monocytogenesΔagrD::pIMK2agrD | pIMK2agrD integrated into the tRNAArg locus in the EGD-e chromosome, Kanr | Riedel et al., 2009 |
| L. monocytogenes EGD-e::pPL2luxPII | pPL2luxABCDEPII integrated into the tRNAArg locus in the EGD-e chromosome, Cmr | This study |
| L. monocytogenes EGD-e ΔagrD::pPL2luxPII | pPL2luxPII integrated into the tRNAArg locus in the EGD-eΔagrD chromosome, Cmr | This study |
| L. monocytogenes EGD-e ΔagrD pNZ44agrBD | Strain with constitutive, P44-driven expression of agrB and agrD, Cmr | This study |
| Plasmids | ||
| pPL2lux | Site-specific integrative vector to study promotor activity in L. monocytogenes, Cmr | Bron et al., 2006 |
| pPL2luxPII | Site-specific integrative vector for PII promoter activity analysis, Cmr | This study |
| pNZ44 | Plasmid for constitutive gene expression driven from the lactococcal promoter P44 | McGrath et al., 2001 |
| pNZ44agrBD | Plasmid for constitutive P44-driven expression of agrB and agrD in L. monocytogenes, Cmr | This study |
| pET29a(+) | Plasmid for strong IPTG inducible expression in E. coli, Kanr | Merck Millipore |
| pET29a_agrB | IPTG-inducible expression of agrB in E. coli, Kanr | This study |
| pET29a_agrBD | IPTG-inducible expression of agrBD in E. coli, Kanr | This study |