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Schematic picture of the experimental design. (A) The following experimental procedures were optimized: seeding of SH-SY5Y neuronal-like cells in 3D scaffolds, oxidative damage in 3D cell-seeded scaffolds by a mitochondrial-targeted toxin (6-OHDA), evaluation of cell metabolic activity while in the 4D bioreactor; (B) Exosome production and characterization.
