FIGURE 3.

Immunodetection of ∼14 kD protein and gene expression of PR1b1 gene. Soluble proteins were isolated from both non-chilled and chilled fruits of azygous and transgenic lines, and Q-PCR analysis of PR1b1 transcripts was carried out RNA isolated from chilled samples following their transfer to rewarming temperatiure of 20°C. (A) Immunoblot analysis with anti-PR1abc antibody of soluble proteins. An arrow points to the immunodetected ∼14 kD protein. Other details were the same as described in the legend to Figure 1. (B) Q-PCR analysis of PR1b1 at different days after rewarming. The transcripts expression levels were determined relative to 0 day sample of the calibrator azygous (556AZ) line. Data set was analyzed by Student’s t-test (Microsoft Excel) comparing control 566AZ at 0 day with other treatments. Each bar represents the mean with standard error of three independent replications. P values are represented as ^∗P < 0.05, ^∗∗P < 0.01.