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. 2016 Jun 22;7:182. doi: 10.3389/fphar.2016.00182

FIGURE 6.

FIGURE 6

Promoted neuronal survival and reduced apoptosis in lesioned brains of mice as a result of ARC treatment. (A–C) Survival of neurons in the lesioned cortex of mice were stained with Nissl and anti-NF-M antibodies. (D,E) Apoptotic cells were detected by caspase-3 immunostaining and TUNEL assay on serial sections of the brain at 7 dpi. (B,F) Areas positive for Nissl antibody were revealed by Nssl staining. (C,G) Areas negative for NF-M were revealed by immunohistochemistry. (D,H) Apoptotic neurons were immunostained with anti-caspase-3+ antibodies. (E,I) Apoptotic cells were determined by TUNEL assay. The amount of NF-M expression around the lesions was quantified by measurement of pixel intensity of NF-M immunoreactivity by measurement of pixel intensity of NF-M immunoreactivity using Image J software. The amount of other positive expression around the lesions was quantified by measurement of number of positive cells using ImageJ software. Boxed areas appear at higher magnifications in the insets. Scale bar: 5 mm in A, 50 μm in (B–E). Nuclei were stained with DAPI (blue). All groups were examined within the areas immediately surrounding the lesion. The data are presented as the mean ± SD, n = 6 per group. ##P < 0.01 vs. naive control; ∗P < 0.05, ∗∗P < 0.01, vs. SWI control.