Figure 2.
Activity-dependent regulation of locus-specific m6A in cultured neurons is associated with mRNA stability. A, MeRIP-qPCR analyses of KCl-depolarized neurons (50 mm, 30 min) showing modulation of locus-specific m6A (n = 3–10; Mann–Whitney U test; *p < 0.05 vs −KCl control). B, qPCR analysis of Fto in neurons transduced with FG12-FTO shRNA [knockdown (KD)], FG12 control, and FUW-FTO [overexpression (OX); n = 8–14; Mann–Whitney U test; ****p < 0.0001]. C, Representative plots showing mRNA decay profiles of Rab33b, Arhgef17, and Crtc1 in actinomycin-D pulse-chase experiments. D, mRNA decay time constant, τ for each transcript (n = 4–14, one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001). E, Decay plots for Egr1 and the calculated τ for Egr1 and Arc (n.s., not significant). Data represent mean ± SEM.