Figure 4.

Electrical stimulation of raphe enhances inhalation-driven glutamatergic input to juxtaglomerular interneurons. A, Raphe stimulation elicits an increase in the magnitude of inhalation-linked iGluSnFR transients imaged from GAD2+ neurons during inhalation of ambient air. Traces show time course of iGluSnFR signal with no stimulation (top trace), a 20 pulse stimulus train (middle trace) and an 80 pulse train (bottom trace). Response map at top shows map of fluorescence increase at the end of the 80 pulse train. White square indicates ROI from which traces are taken. B, Raphe stimulation causes a similar increase in iGluSnFR signals imaged from TH+ neurons, with increasing effect size and duration with a longer (80 pulse) stimulus train.