Figure 4.

The luminescence intensity at 545 nm of Tb^III-DOTAM (blue bars) and Tb^III ₂-L¹ (red bars) in the presence of various phosphorylated and nonphosphorylated amino acids, nucleoside derivatives, and PhOP (a model compound of pTyr). Conditions: [Tb^III complex] = [additive] = 100 μM, pH 7.0 (10 mM HEPES buffer), λ _ex = 262.5 nm. For the purpose of comparison, the results using Tb^III ion without ligand are also presented (yellow bars). Note that nucleotides (UMP, GMP, CMP, and ADP) showed notable signals and thus selective detection of pTyr was unsuccessful.