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. 2016 Jun 14;7(3):e00618-16. doi: 10.1128/mBio.00618-16

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Copyright © 2016 Rivera-Hernandez et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Indirect bactericidal assay. Pooled heat-inactivated sera raised against vaccine antigens in BALB/c (A) and humanized plasminogen AlbPLG1 (B) mice were preincubated with GAS pM1.200 and 5448, respectively. Human blood tested to support the growth of both strains was then added, and following 3-h incubation at 37°C, samples were plated for CFU determination. The survival percentage was calculated using the growth of GAS in serum from PBS-immunized mice as 100%. Values are the mean survival percentage plus standard deviation (SD) (error bars) for three independent replicates. Survival percentages were compared using one-way ANOVA corrected for multiple comparisons using Dunnett’s test. Values that are significantly different from the value for the control (α PBS) are indicated by asterisks as follows: **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.