FIG 2 .

Anoxic growth is important for A. actinomycetemcomitans monoinfection. (A) Venn diagrams showing the overlap between monoinfection and anoxic (left) or oxic (right) growth in vitro. The percentages represent the percentage of anoxic (left) or oxic (right) fitness determinants that overlap monoinfection fitness determinants. The P values represent the significance of the enrichment of anoxic (left) or oxic (right) fitness determinants (one-tailed Fisher exact test). (B) Spearman’s rank correlation (r) between P values for the enrichment of COGs among monoinfection and anoxic (left) or oxic (right) fitness determinants (one-tailed Fisher exact test). Each point corresponds to an individual COG. (C) Biosynthetic requirements for monoinfection determined as described in the materials and methods. Metabolites in blue and orange are required for anoxic and oxic growth, respectively. PABA, p-aminobenzoic acid; SAM, S-adenosylmethionine; PLP, pyridoxal phosphate. See Table S5 in Dataset S1 in the supplemental material for a full summary. (D) Central metabolic pathways required for monoinfection. Green and red indicate pathways required for growth in the abscess and growth in vitro, respectively. Abbreviations not defined here are defined in the legend to Fig. 1 or in the text.