FIG 5 .

Metabotyping of blood serum from F₂ Gif^tm1a/tm1a and wild-type mice reveals metabolic abnormalities. (a) PCA score plot separating naive F₂ Gif^tm1a/tm1a mice (green circles) from naive wild-type mice (blue circles) with 92% of the variables explained (R²_cum) by using the first four components with 72% predictability minus Q2. (b) Multivariate data analysis results comparing the blood serum of uninfected F₂ Gif^tm1a/tm1a (ui_gif; red circles) and wild-type (ui_wt; yellow circles) mice and that of S. Typhimurium-infected F₂ Gif^tm1a/tm1a (si_gif; green circles) and wild-type (si_wt; blue circles) mice. Principal component 1 discriminates on the basis of the presence or absence of infection, while principal component 2 separates different mouse genotypes, regardless of infection status. Ninety percent of the variables (R²_cum) were explained by using the first five components with 73% predictability.