Fig. 3.

Callus formation capability of the toc1-3 mutant. (A) Callus formation. Leaf explants from third-leaves of two-week-old plants were used to induce calli on CIM (n > 30). Plates were incubated for 2 weeks under continuous dark conditions and photographed. Scale bars = 5 mm. (B) Fresh weight measurement. Thirty calli were collected to measure fresh weight. Bars indicate the standard error of the mean. Statistically significant differences between wildtype and toc1-3 mutant are indicated by asterisks (Student’s t-test, ^*P < 0.05).