Fig. 1.

Multiple sequence alignment of EcEIIB^fruc and its homologues. Abbreviations are as follows: Predicted EIIB^fruc molecules from Escherichia coli str. K-12 substr. MG1655 (EcEIIB(fruc)), Klebsiella pneumoniae 342 (KpEIIB(fruc)), Streptococcus bovis ATCC 700338 (SbEIIB(fruc)), Bacillus Subtilis Subsp. Subtilis Str. 168 (2R48), Listeria monocytogenes EGD-e (2M1Z), Pelosinus fermentans DSM 17108 (PfEIIB(fruc)), E. coli K-12 (2KYR), Bacillus subtilis subsp. subtilis str. 168 (2R4Q), E. coli UTI89 (YpdH), EcIIB^cel (EcIIB(cel)), GatB E. coli from (1TVM), PtxB from Streptococcus (3C2Z) and the cytoplasmic B domain of the mannitol transporter IIABC from E. coli (1VKR). Secondary structure elements of EcEIIB^fruc, the GOT model and EcIIB^cel are drawn below the sequences and colored red for α-helix, green for β-strand and yellow for 3₁₀-helix. The dotted lines represent the residues missing in the coordinates. The highly conserved motifs are indicated. The conserved sequences at the P-like loop motif are marked with a red box. The red characters represent the key residues for transferring a phosphoryl group. Glu38 and Arg73 forming a salt bridge are marked as the blue characters. The consensus amino acids are colored according to the Clustal X color scheme provided by the Jalview program (Waterhouse et al., 2009).