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. 2016 Jun 22;6:28287. doi: 10.1038/srep28287

Figure 2. Influenza-specific effector cells distribute between Tfh and NonTfh depending on epitope specificity.

Figure 2

Single epitope reactivity of sorted CD4 T cell populations at day 9 post infection, Tfh (Filled) and NonTfh (Open) from mediastinal lymph nodes pooled from 20–25 mice. CD4 T cell populations were assayed for epitope reactivity in IL-2 ELISPOT assays by restimulation with influenza-derived epitopes from HA (a) and NP (c). The epitope-specific IL-2 spots per million Tfh or NonTfh cells are shown. Relative distribution of antigen-reactive CD4 T cells between Tfh and NonTfh cells is depicted by the ratio of antigen-specific cells in the two populations for HA-specific T cells (b) and NP-specific cells (d). Mean and S.D. of 4 experiments. Statistical significance determined by two-tailed paired t test (a,c) and 1-way ANOVA with Tukey post test (b,d). *p < 0.05; **p < 0.01.