Figure 4. Epitope locations within the native protein context of hemagglutinin and nucleoprotein and the heterologous protein MalE133.

Protein structures of MalE133 (a), hemagglutinin (b) and nucleoprotein (c). Influenza epitopes and their locations are indicated by coloration and are denoted by first amino acid associated with the 17-mer peptide used for testing of CD4 T cell reactivity. For MalE133 protein, a heterologous peptide is shown for illustrative purposes at the insertion site within the protein structure. The structures used to model peptide epitope location were sufficiently homologous to H1N1 A/New Caledonia/20/1999 proteins to overlay New Caledonia epitopes: Hemagglutinin (HA0) from A/South Carolina/1/18 (H1N1); Nucleoprotein from A/England/256/2009 (H1N1).