Skip to main content
. 2016 Jun 22;6:28497. doi: 10.1038/srep28497

Figure 7. CH-1a nsp4 has no effect on VISA cleavage.

Figure 7

(A–C) nsp4 of JXwn06 or CH-1a isolate expression plasmid was transfected into 3D4/21 cells along with pGL3-IFNβ-Luc (A), pGL3-IRFs-Luc (B) or pGL3-NF-κB-Luc (C), and pRL-TK. pRL-TK was used as an internal control of transfection efficiency. Twenty-four hours later, cells were stimulated with or without poly(I:C) (10 μg/ml) for 8 h and analyzed using dual-luciferase assay. (D) PAMs were mock infected or infected with JXwn06 or CH-1a at an MOI of 1 for 24 h, and cells were harvested and performed the expression of VISA and nsp4 by Western blot analysis. (E) 3D4/21 cells were transfected with JXwn06 nsp4 or CH-1a nsp4 expression plasmid for 24 h, and then cell lysates were analyzed by Western blotting with antibodies for VISA, nsp4 and β-actin. β-actin was set up as a loading control. (F) HeLa cell treatment and confocal microscopy were performed as described in the legend to Fig. 7B. Data are mean ± SD from three independent experiments. Differences were evaluated by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.