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. 2016 Jun 22;6:28317. doi: 10.1038/srep28317

Table 2. Dazl depletion blocks PGC formation1.

Serum injected Number of embryos Number of PGCs
    Total Side 12 Side 22
Preserum 25 33.5 ± 5.3 14.5 ± 3.2 19.0 ± 3.1
αDazl 43 23.3 ± 6.4 7.9 ± 3.5 15.4 ± 4.6

1Preserum or αDazl was injected with 1.5 ng per NgVg embryo into one of the two cells at the 2-cell stage, and PGCs were scored by GFP expression at 40 hpf.

2Side 1 is the injected side, which was labeled by a co-injected fluorescent dye, and side 2 is the noninjected side. Significant difference was observed between preserum injection and αDazl injection in the injected side but not noninjected side.