Table 2. Dazl depletion blocks PGC formation1.
Serum injected | Number of embryos | Number of PGCs | ||
---|---|---|---|---|
Total | Side 12 | Side 22 | ||
Preserum | 25 | 33.5 ± 5.3 | 14.5 ± 3.2 | 19.0 ± 3.1 |
αDazl | 43 | 23.3 ± 6.4 | 7.9 ± 3.5 | 15.4 ± 4.6 |
1Preserum or αDazl was injected with 1.5 ng per NgVg embryo into one of the two cells at the 2-cell stage, and PGCs were scored by GFP expression at 40 hpf.
2Side 1 is the injected side, which was labeled by a co-injected fluorescent dye, and side 2 is the noninjected side. Significant difference was observed between preserum injection and αDazl injection in the injected side but not noninjected side.