Table 2. Analytical parameters for the individual substrate, metabolite and internal standard (IS).

Analyte	Ionization mode	Precursor ion (m/z)	Product ion (m/z)	Fragmentor (V)	Collision energy (eV)
Phenacetin	ESI+	180.1	110.2	110	21
4-acetamidophenol	ESI+	152.1	110.1	90	15
Bupropion	ESI+	240.1	184.1	90	8
Hydroxybupropion	ESI+	256.1	238.1	70	8
Tolbutamide	ESI−	269.2	170.0	100	12
4-hydroxytolbutamide	ESI−	285.2	186.0	100	12
Dextromethorphan	ESI+	272.3	147.1	150	33
Dextrorphan	ESI+	258.1	157.1	150	42
Chlorzoxazone	ESI−	168.0	132.0	110	20
6-hydroxychlorzoxazone	ESI−	184.1	120.0	90	17
Midazolam	ESI+	326.1	291.0	170	28
1-hydroxymidazolam	ESI+	342.1	324.0	130	20
3-acetamidophenol (IS)	ESI+	152.1	93.0	80	26
Chlorpropamide (IS)	ESI−	275.1	190	100	11
Mebendazole (IS)	ESI+	296.1	264.1	150	22