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. 2016 Jun 22;6:28481. doi: 10.1038/srep28481

Figure 3. Examination of TNF-α stimulated IκBα degradation and phosporylation in NS1 and NS1-70-expressing cells.

Figure 3

293T cells transfected with empty vector, NS1 or NS1-70-expressing plasmid were stimulated with TNF-α (20 ng/ml) for indicated durations. Equal amounts of cell lysates were analyzed by immunoblotting with the anti-IκBα antibody or the anti-phospho-IκBα antibody. The results represent three independent experiments. The protein levels of NS1 and NS1-70 as well as β-actin in the same cell lysates were determined by immunoblotting.