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. 2016 Jun 22;6:28481. doi: 10.1038/srep28481

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Copyright © 2016, Macmillan Publishers Limited

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293T cells were transfected separately with NS1 (NS1-HA or NS1-FLAG) or NS1-70 (NS1-70-HA or NS1-70-FLAG) or either p65 1-190-β-gal (p65 1-190-β-gal -HA or p65 1-190-β-gal-FLAG) or β-gal (β-gal-HA or β-gal-FLAG) expression plasmids for 30 h. All cells were then treated with TNF-α (10 ng/ml) for 30 min. The cells were lysed and subjected to immunoprecipitation (IP) using the mouse anti-FLAG tag. Mouse IgG was used as negative control. IP products and 5% input samples were analyzed by immunoblotting. For detection of FLAG-tagged and HA-tagged proteins, the indicated mouse Abs were used. One of three experiments is shown.