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. 2016 Jun 22;6:28481. doi: 10.1038/srep28481

Figure 8. HBoV NS1, rather than NS1-70, suppresses p65 phosphorylation.

Figure 8

293T cells were transfected with NS1 or NS1-70 expression plasmid or empty vector for 30 h. The cells were then mock-treated or treated with TNF-α (20 ng/ml) for 15 min. Equal amounts of cell lysates were analyzed by immunoblotting with anti-p-p65 (Ser536) antibody or anti–p65 antibody. The results represent three independent experiments. The protein levels of NS1-HA, NS1-70-HA, and β-actin in the same cell lysates were determined by immunoblotting.