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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: J Cell Biochem. 2015 Nov 26;117(6):1429–1438. doi: 10.1002/jcb.25433

Figure 1. Expression of mouse BRDT in 293T cell lines.

Figure 1

(A) Analysis of ectopically expressed BRDT in selected 293T clones. Approximately 20 μg of whole extract isolated from either 293T, 293T/pBABE, or 293T/BRDT-FLAG cells was analyzed by immunoblotting using either anti-FLAG, BRDT, or GAPDH rabbit polyclonal antibodies. (B) Nuclear extracts from 293T/pBABE and 293T/BRDT-FLAG cells were FLAG-immunoprecipitated, and after extensive washing the retained proteins were analyzed by immunoblotting using anti-BRDT and anti-FLAG antibodies. (C) Immunoblot analysis of BRDT-FLAG nuclear and cytoplasmic expression in one of the stable 293T/BRDT-FLAG cell lines. Detection of tubulin served as a cytoplasmic extract control.