Table 2. Primers used for double stranded RNA synthesis.
| Primer Name | Forwad Primer (5′-3′) | Reverse Primer (5′-3′) | Length (bp) |
|---|---|---|---|
| NLTRE1-1 | ATGTCCCAATGTGCCATTCACAG | TCACGTACCATTCAAGAATATGTC | 1803 |
| DSNLTRE1-1 | GATGCAATCAAGGAGGTGTTATGGC | CGTATTCACCTCCACCTCCGT | 451 |
| DSNLTRE1-1-T7 | T7-GATGCAATCAAGGAGGTGTTATGGC | T7-CGTATTCACCTCCACCTCCGT | |
| NLTRE1-2 | ATGAAGGCAAAAAAACATGGTGAGGCC | CTATAAATGATGCATGAAACGTTTTTCC | 1506 |
| DSNLTRE1-2 | AGATGAAGGCATGTGGTTCG | CATCGATTCGCCAACTGGTAAGC | 321 |
| DSNLTRE1-2-T7 | T7-AGATGAAGGCATGTGGTTCG | T7- CATCGATTCGCCAACTGGTAAGC | |
| NLTRE2 | ATGACGACTGTTAATCTCTTCACGGT | CTAGTCACATGGTTTTAGATCCTTC | 1998 |
| DSNLTRE2 | CCAACTGCTATGACACCGACAAG | GGGTTCAGATCCTGCCGTCGCT | 440 |
| DSNLTRE2-T7 | T7-CCAACTGCTATGACACCGACAAG | T7-GGGTTCAGATCCTGCCGTCGCT | |
| DSNLGFP | AAGGGCGAGGAGCTGTTCACCG | CAGCAGGACCATGTGATCGCGC | 688 |
| DSNLGFP-T7 | T7-AAGGGCGAGGAGCTGTTCACCG | T7-CAGCAGGACCATGTGATCGCGC |
T7 sequence: GGATCCTAATACGACTCACTATAGG.