Table 3. Primers used for quantitative real-time PCR (qRT-PCR) of genes involved in the chitin metabolism pathway.

Gene	Genebank number	Forwad (5′-3′)	Reverse (5′-3′)
NLTRE1-1	FJ790319	GCCATTGTGGACAGGGTG	CGGTATGAACGAATAGAGCC
NLTRE1-2	KU556829	GATCGCACGGATGTTTA	AATGGCGTTCAAGTCAA
NLTRE2	GQ397451	TCACGGTTGTCCAAGTCT	TGTTTCGTTTCGGCTGT
NLHK	KU556830	GGTGCGAGAAGAAGTGAAG	GTGAAACCCATTGGTAGAGT
NLGPI1	KU556831	CCTGCCACCAGTCATAACCC	CTTGTGAGGAAGGATGCGTTT
NLGPI2	KU556832	ATCAGCCGTAGCCAAGCAC	AAGCCGATAGCAGACCACAAC
NLGFAT	KU556833	CCTCCCAGTTCATCTCGC	CCAAGTTCTTCAAACCCTTTAT
NLGNPNA	KU556834	TGAGCTGCTGAAGACACT	CCTGAATAACGGTGATGTA
NLPGM1	JF330414	AACGAGACGGTGGGAGAC	TCCTGGTAAGTGTTGAGCC
NLPGM2	KU556835	AGAGGAAGGTTGGGAGTG	CATAATTCGCGGAGATAAG
NLUAP	JF330415	ACGACAGATTAAAGCCGATAC	TACCTTGTCCACCAGCCA
NLCHS1	AEL88648	CCGCAAACGATTCCTACAGA	AGGTCCTTGACGCTCATTCC
NLCHS1a	JQ040014	TGTTCTTGCTACAACTCAATAAA	ACACCAATCCGATAGGCTC
NLCHS1b	JQ040013	GCTGTCTTTGCTTTCTTCAT	ACACCAATCCGATAGGCTC
NLCht1	AJO25036	AGGTGGTTAGGGACGAGGAG	TGCGCTTGACATAGTTGGACT
NLCht2	AJO25037	GCAGATTTCTGGACAGGGAA	TGACGCACAAGCGGGAAG
NLCht3	AJO25038	CTACACCTCTGGCTAAACTCGG	AACTTGTCCTTGCGGCTGAT
NLCht4	AJO25039	TTGAGGAGGTTCACGGGTCT	CCTTACTGGAAACGAGGTTGG
NLCht5	AJO25040	AAAGCGTTCGTGATGAAATAGC	GATCCTTTGCCTCAATCCAAT
NLCht6	AJO25041	GCTGGTAAGGAGATGCTATTCG	GTGGTTCTAAGGCTGGCTGTC
NLCht7	AJO25042	CTACTCTGCCATCCCATTCCT	GTCTGGGTTTCTTCACTTCCTG
NLCht8	AJO25043	GAACAAAGTGCAAACTCAGTCC	CACCTTCTGTGGCTTCTGG
NLCht9	AJO25044	GTGCGGTATTGGTTGAAGAGG	GGTATAACGTGATTCCGAGCC
NLCht10	AJO25045	CAAGCCAATACCCAACAAAC	ACAGCAAATCCATAGAGCACA
NLIDGF	AJO25056	AAAAGAACGAGGAGGAGGG	TTGCTTGAGGATGGGGTAC
NLENGase	AJO25057	TGTGGCAAGACTTCGTTA	ATGGGAGGGTTGGGATAG
NL-18S	–	CGCTACTACCGATTGAA	GGAAACCTTGTTACGACTT