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. 2016 Jun 22;6:28403. doi: 10.1038/srep28403

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(a) Cells were treated with 0.4 μM dioscin for 24 h and cellular morphology was observed by phase-contrast microscopy. Vacuoles in the cytoplasm were marked by arrowhead. Magnification: 400×. LC3-II and beclin-1 protein expression was determined by Western blotting (b). Means ± SD of three experiments are presented.***p < 0.001 versus that obtained in control group.