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. 2016 Jun 22;6:28403. doi: 10.1038/srep28403

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(a–c) Effect of dioscin (0.4 μM) on the levels of phosphorylation of PI3K and Akt in MCF-7 and MCF-7/ADR cells. (d,e) Effect of dioscin and/or PI3K/AKT inhibitor LY294002 on the levels of LC3 protein expression in MCF-7 and MCF-7/ADR cells. Cells were treated with dioscin (0.4 μM) or LY294002 (20 μM) for 24 h. Cell lysates were prepared and analyzed by Western blot analysis. ***p < 0.001, **p < 0.01 versus that obtained in the corresponding control group.