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. 2016 Jun 22;6:28377. doi: 10.1038/srep28377

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Copyright © 2016, Macmillan Publishers Limited

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NUCB1 mRNA expression in goldfish hypothalamus after 7 days (a) or 28 days (b) of feeding special diets containing varying amounts of macronutrients. NUCB1 mRNA expression in goldfish hypothalamus was downregulated in response to high carbohydrate and very high fat (20%) feeding for 7 days (a). NUCB1 mRNA expression was significantly reduced in goldfish hypothalamus post- high carbohydrate and high fat (9% and 20%) feeding for 28 days (b). In gut, NUCB1 mRNA expression was attenuated after high carbohydrate and fat (9%, 20%) after 7 days (c). Meanwhile, NUCB1 mRNA expression was decreased in the gut of goldfish fed on high protein, high carbohydrate and fat (20%) diet after 28 days of feeding (d). NUCB1 mRNA expression were normalized to beta-actin. Asterisks denote significant differences between treatment groups (p < 0.05, n = 8 fish/group). Data are represented as mean + SEM. One-way ANOVA (non-parametric) followed by Tukey’s multiple comparison test were used for statistical analysis.