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. 2016 Jun 22;6:28377. doi: 10.1038/srep28377

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression of NUCB1 mRNA expression in the gut (a), forebrain (b), hindbrain (c), hypothalamus (d) and pituitary (e) of goldfish maintained under 12L: 12D photoperiod and scheduled feeding. The mRNA expression data was normalized to beta-actin. Data are presented as mean + SEM. The grey areas represent the night time and arrows indicate feeding time. Different alphabets denote significant differences between the time points (P < 0.05, n = 6 fish/group). One-way ANOVA (non-parametric) followed by Tukey’s multiple comparison test was used for statistical analysis. The dashed line in figure b indicates a significant rhythm determined by cosinor analysis.