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. 2016 Jun 22;6:28377. doi: 10.1038/srep28377

Figure 5.

Figure 5

Estradiol (100 μg/g BW) decreased NUCB1 mRNA expression in female goldfish pituitary (a). Testosterone (100 μg/g BW) increased NUCB1 mRNA expression in the gut, hindbrain, and hypothalamus of female goldfish (b). Expression of NUCB1 in gut, forebrain, hindbrain, hypothalamus and pituitary of fish treated with estradiol (a) or testosterone (b) were normalized to control group. The mRNA expression data were normalized to 18 s RNA. Asterisks denote significant differences between the experimental groups. (*p < 0.05, **p < 0.01, ***p < 0.005, n = 7 female goldfish/group). Food deprivation decreased relative mRNA expression of NUCB1 in goldfish tissues after 3 days (c) and 7 days of food deprivation (d). The mRNA expression data were normalized to 18 s RNA. Asterisks denote significant differences between control and fasted groups (*p < 0.05, n = 6 fish/group). Data is represented as mean + SEM. One-way ANOVA (non-parametric) followed by Tukey’s multiple comparison test and Student-Newman-Keuls test (a, b) were used for statistical analysis.