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. 2016 Jun 22;6:28377. doi: 10.1038/srep28377

Figure 7.

Figure 7

Goldfish NLP (1, 10, 100 ng/g BW) downregulated preproghrelin (a) and orexin-A (b) mRNA expression in the hypothalamus of goldfish when compared to saline treated control (denoted by 0), while upregulated CART mRNA expression at the highest dosage (100 ng/g BW) of NLP tested (c). Similarly, goldfish NLP (1, 10, 100 ng/g BW) also reduced preproghrelin (d) and orexin-A (e) mRNA expression, and upregulated CART mRNA expression at the highest dosage of NLP (100 ng/g BW) in the gut of goldfish (f). Intraperitoneal injection of rat NLP downregulated preproghrelin (g) and orexin-A (h) mRNA expression in the hypothalamus of goldfish. No significant change in CART mRNA expression in the hypothalamus of goldfish was found (i). The mRNA expression data was normalized to 18 s RNA. Asterisks denote significant differences between control and NLP injected groups (*p < 0.05, n = 10 fish/group). Data are presented as mean + SEM. One-way ANOVA (non-parametric) followed by Tukey’s multiple comparison test was used for statistical analysis.