Table 1.
Protective effect of ARBME and ARBWF against CCl4 intoxicated HepG2 cell lines.
| Group no. | Treatment | % Cell viability | LDH (U/L) | |
|---|---|---|---|---|
| 1 | Group-I | Normal control | 99.42 ± 0.26 | 96.64 ± 1.67 |
| DMSO control (0.25% V/V) | 98.19 ± 0.18 | 95.47 ± 1.45 | ||
| Silymarin control (200 μg/ml) | 98.56 ± 0.21 | 97.18 ± 1.58 | ||
| ARBME (200 μg/ml) control | 98.07 ± 0.19 | 97.73 ± 1.76 | ||
| ARBWF (200 μg/ml) control | 97.84 ± 0.22 | 96.29 ± 1.41 | ||
| 2 | Group-II | Toxin CCl4 control (1% v/v) | 19.72 ± 0.26∧ | 197.64 ± 3.38 |
| 3 | Group-III | Silymarin 50 μg/ml + CCl4 (1% v/v) | 68.22 ± 0.21∗ | 123.37 ± 2.19∗ |
| Silymarin 100 μg/ml + CCl4 (1% v/v) | 76.47 ± 0.18∗ | 115.42 ± 2.35∗ | ||
| Silymarin 200 μg/ml + CCl4 (1% v/v) | 88.39 ± 0.24∗ | 106.42 ± 1.84∗ | ||
| 4 | Group-IV | ARBME 50 μg/ml + CCl4 (1% v/v) | 39.64 ± 1.20∗ | 182.71 ± 2.46∗ |
| ARBME 100 μg/ml + CCl4 (1% v/v) | 45.27 ± 1.23∗ | 159.65 ± 2.21∗ | ||
| ARBME 200 μg/ml + CCl4 (1% v/v) | 52.66 ± 1.17∗ | 142.56 ± 1.87∗ | ||
| 5 | Group-V | ARBWF 50 μg/ml + CCl4 (1% v/v) | 47.25 ± 1.26∗ | 151.19 ± 1.49∗ |
| ARBWF 100 μg/ml + CCl4 (1% v/v) | 56.17 ± 1.21∗ | 138.24 ± 2.17∗ | ||
| ARBWF 200 μg/ml + CCl4 (1% v/v) | 64.42 ± 0.95∗ | 129.68 ± 1.41∗ |
All the results were expressed in mean ± SD (n = 3). ∧P < 0.05 in comparison of group-II with group-I. ∗P < 0.05 in comparison of groups-III, IV, and V with group-II. ARBME, A. reticulata bark methanol extract; ARBWF, A. reticulata bark water fraction.