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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: J Addict Res Ther. 2016 Apr 7;7(2):274. doi: 10.4172/2155-6105.1000274

Figure 5.

Figure 5

Double immunofluorescence laser scanning confocal microscopy images demonstrating the location of nicotine (NIC), and nicotine pyrrolidine methiodide (NIC-PM) induced c-Fos immunoreactive (IR) neurons with respect to tyrosine hydroxylase (TH) IR DA11 cells in posterior hypothalamus. Panels A–C: Control data demonstrating effects of acute intraperitoneal injection of physiological saline (PS) on c-Fos activation of TH IR DA11 cells. Panels D-F and G-I: NIC and NIC-PM data respectively, showing c-Fos IR cells (D, G), TH IR cells (E, H) and merge images of c-Fos with TH IR DA11 cells (F, I). Arrows point to areas magnified in photomicrographs.