Table1.
Properties and amounts of primers used in real-time RT-PCR assays of marker and reference genes
| mRNA Marker | Accession number |
Forward primer
5' → 3' |
Reverse primer
5' → 3' |
Amplicon length (bp) |
|---|---|---|---|---|
| GCC | NM-004963 | GGGTGGCTGTCCTTTAGTT | GTAGCGTTCACAGTCACA | 191 |
| CK19 | NM-002276 | TCCGAACCAAGTTTGAGAC | AATCCACCTCCACACTGA | 222 |
| CK20 | NM-019010 | ACGCCAGAACAACGAATACC | TTCAGATGACACGACCTTGC | 208 |
| 18S rRNA | X03205 | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | 151 |
GCC: Guanylyl Cyclase C
CK19: Cytokeratin 19
CK20: Cytokeratin 20
18S rRNA: 18S ribosomal RNA