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Agarose gel electrophoresis analysis of recombinant pET28a/fliC with restriction enzyme digestion. Lane M; DNA molecular weight marker (1 kb); lane 1: mono-digestion of the pET28a/fliC vector with BamHI. One expected fragment was observed on the gel (~ 6836 bp band). Lane 2: BamHI/HindIII digested the recombinant vector. Two expected fragments from double digestion were observed on the gel (~ 5369 and 1467 bp bands). Lane 3; the optimized PCR product of the fliC gene (~ 1467 bp band
