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SDS-PAGE analysis of the expression of r-B-flagellin protein in E. coli. Lane M: molecular weight marker proteins; lanes 1-6: 1-6 h incubation after induction; lane 7: 21 h incubation after induction; lane 8: purified r-B-flagellin after HisTrap Chelating and Ni2+-affinity chromatography. The arrow indicates the position of the type B-flagellin (~ 53 kDa
