Skip to main content
. 2015 Nov 20;5(2):175–185. doi: 10.1080/21623945.2015.1111972

Figure 3.

Figure 3.

Effect of reverse siRNA transfection on brown adipocyte function. Mature WT-1 adipocytes reverse transfected with Pkm siRNA or universal negative siRNA at day 6 of adipocyte differentiation and harvested 4 d later. Relative mRNA expression levels (measured by RT-qPCR) of (A) Pkm, (C) Fabp4, and (D) Glut4 were determined by normalization to expression levels of Tbp. Data represent mean of means +SEM (n = 3). *, p < 0.05 vs. universal negative siRNA. (B) Immunoblotting analyses of Ser473-phosphorylated AKT (p-AKT) and PKM following stimulation with 5 μg/ml insulin for 15 or 30 min. GAPDH was used as a loading control (n = 2). (E) Cell culture medium glycerol content from reverse transfected cells stimulated with 1 μM isoproterenol for 6 h, depicted as fold increase compared to non-stimulated cells. Data represent mean of means +SEM (n = 3).